FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_6331.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_6331.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17774129
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA43100.058.7195666
TCGGAGA51600.052.0050358
CGGAGAT53850.050.508669
CTCGGAG56200.045.6816677
GGCTCGG54550.041.3410955
GGGCTCG44800.040.298494
CGTGGGC55700.020.2998961
TATACTG196700.016.1836515
TACACCG22650.015.367015
TGACGCG21400.014.899039145
GCCGACC37350.014.744918145
CTATACT134250.014.6900084
CCTACAC83400.014.4297693
TACACTG162050.014.0059195
GATCGGA16650.013.93703656
GTATAGA130850.013.903511
CTACACT116450.013.88458254
GTATAGG69050.013.3310541
TACACAG197350.013.227625
GTATTAG128700.013.1783711
Produced by FastQC (version 0.11.5)