FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_4329.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_4329.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17886525
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA81450.051.2797436
CTCGGAG96200.043.5703967
CGGAGAT104250.043.5491339
TCGGAGA106500.041.809698
GGCTCGG86750.041.123325
GGGCTCG79600.037.3438954
TGGGCTC146550.020.6769773
CGTGGGC115250.017.2319091
TACACTG120800.017.1668935
GTGGGCT125300.016.0825122
TATACTG176850.015.7031325
TACACCG72000.015.2067725
GTGTAGG105600.015.1001941
CTATACT130750.014.63898854
CTACACT107350.013.6426324
TATACCG51950.013.5387555
CCTATAC85850.012.9195613
TACACAG153600.012.8873615
CCTACAC98550.012.7258293
TAGACTG88950.012.7166065
Produced by FastQC (version 0.11.5)