FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_341.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_341.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14396135
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA29350.049.4115756
CGGAGAT37450.040.4732179
TCGGAGA36950.039.841188
CTCGGAG41000.035.5502937
GGCTCGG38950.032.391465
GGGCTCG33400.029.304774
TATACTG168350.016.2374175
TACACTG128400.015.1341665
CCTATAC57600.014.8516873
GTATAGG59950.014.3877911
GTCCTAC46000.014.3390331
TACACAG165350.013.9009285
GTATAGA111850.013.8032051
GTGTACG16300.013.7851151
GTATTAG106500.013.61183551
CTATACT105500.013.538324
GTGTAAG95550.013.4269981
CTACACT97250.013.4194244
CTTACAC99300.013.3603593
CCTACAC71800.013.1260843
Produced by FastQC (version 0.11.5)