FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_31013.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_31013.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16408104
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA46100.066.382426
CGGAGAT54150.062.281969
TCGGAGA53250.060.7448928
CTCGGAG62600.048.7728847
GGCTCGG53850.048.7479635
GGGCTCG44150.046.642754
CGTGGGC55100.024.5984541
TATACTG189750.017.0828115
CTATACT115450.015.4503274
GTATAGA119300.015.4316031
TACACTG140650.014.6939555
CTAGGAC72200.014.4594463
GTGTAGG80100.014.2064281
TGACGCG26050.013.908643145
CTTACAC102500.013.8630433
GTGTTAG126600.013.7402721
GTATAGG63300.013.7402721
CCGTATA32900.013.655835145
GTGTAAG110050.013.6332091
CCTATAC61450.013.5675773
Produced by FastQC (version 0.11.5)