FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_30981.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_30981.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24041603
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA92100.090.072386
TCGGAGA101650.086.472398
CGGAGAT105200.084.937569
GGCTCGG96200.075.601815
CTCGGAG114300.072.709747
GGGCTCG80550.071.6465154
CGTGGGC77900.046.800811
GATCGGA20700.026.2733966
CGGCTCG19800.022.3363484
TGGCTCG50950.022.0564214
GTGGGCT192050.020.3066272
ATCGGAG29700.020.2663637
TGGGCTC295600.020.1839793
TGACGCG24000.019.323826145
TGCTCGG44700.018.9794945
GCACGGA36200.017.4275346
GCCGACC53400.017.23403145
GCTCCGA26950.016.9514566
ACGAACA80050.015.479579145
ACGAACG25100.015.301237145
Produced by FastQC (version 0.11.5)