FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_30981.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_30981.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15609693
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG185260.11868266723759399No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA56700.081.335736
TCGGAGA64250.077.6555948
CGGAGAT67550.077.6234059
GGCTCGG62700.063.719845
GGGCTCG54550.062.0686654
CTCGGAG75650.061.82857
CGTGGGC59800.036.605131
GATCGGA15800.022.9467016
TGGGCTC179000.019.642523
GTGGGCT120750.018.9099432
GCTCCGA22200.016.9846956
ATCGGAG22900.016.7833397
GACGACA48400.016.619493145
TATACTG152150.016.584335
GTGTAGG78150.015.58175851
TACACCG22350.015.5723575
CTATACT104100.014.8347134
CCTATAC58750.014.8075043
GTATAGG60400.014.7606111
TACACTG127100.014.6614935
Produced by FastQC (version 0.11.5)