FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_30979.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_30979.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20096251
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA59600.074.460336
TCGGAGA70500.067.172658
CGGAGAT73150.065.138519
CTCGGAG72850.061.3195577
GGCTCGG70950.053.9610985
GGGCTCG59250.051.884394
CGTGGGC69050.027.187341
GTATAGG81550.015.3763311
TACACTG179000.014.866625
TGGGCTC232150.014.395963
GATCGGA18800.014.2713036
TATACTG218100.014.0299465
GTGGGCT148700.013.94201852
GTATAGA142750.013.6586171
GTGTTAG155050.013.6502841
CTATACT149900.013.6397594
GTAGGAC59650.013.2472113
CTACACT137050.013.1728434
GTATTAG154700.013.1189261
TACACAG229400.012.8331075
Produced by FastQC (version 0.11.5)