FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_30907.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_30907.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14672278
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA51700.060.7322546
CGGAGAT59000.057.4045269
TCGGAGA58200.056.321168
CTCGGAG60700.050.7745367
GGCTCGG61100.044.3840835
GGGCTCG51150.039.8316154
CGTGGGC65700.019.3072321
TACACTG123650.015.8917625
TACACCG29900.015.7630065
TATACTG158200.015.6294895
GTGTAGG73500.014.79282951
GTAGGAC46600.014.0018473
GTACTAG49550.013.8971991
CTATACT109000.013.6362394
CTTACAC90500.013.6184943
TGGGCTC164650.013.5177793
CCGACGC86950.013.502181145
CTACACT96150.013.1964164
GTGCTAG78450.012.9354751
TAGACTG93600.012.782185
Produced by FastQC (version 0.11.5)