FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_30905.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_30905.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17883448
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG182320.10194902012184676No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA64800.046.1045886
CGGAGAT77550.041.4319159
TCGGAGA76400.040.9145288
CTCGGAG78850.037.249697
GGGCTCG64650.032.0739634
GGCTCGG81200.031.8800935
TACACCG55650.015.8965195
TATACTG191250.015.6966255
CGTGGGC90100.015.2843821
CTATACT121550.014.7332014
TACACTG133200.014.4261035
TACACAG162750.013.7671755
TCTATAC129050.013.5946733
CTACACT102300.013.3240834
TAGACTG97700.013.2109125
TGGGCTC169900.013.1848893
GTATAGA126700.012.9285821
CCTACAC85950.012.9049683
TAGACAG194600.012.8926035
GTATTAG124650.012.7923241
Produced by FastQC (version 0.11.5)