FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_30900.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_30900.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18558185
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG233550.1258474360504543No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA74250.080.183096
TCGGAGA85000.074.316988
CGGAGAT88700.073.510129
CTCGGAG91250.064.692957
GGCTCGG80700.063.168125
GGGCTCG70000.058.8326574
CGTGGGC76450.035.459571
TGGGCTC216650.019.6766223
GATCGGA21100.018.9023726
GTGGGCT150850.018.837612
TATACTG213500.017.2197275
TGGCTCG51450.016.2061794
CTATACT134750.015.6578594
TACACTG154300.015.1793365
TCCGAGA35050.014.8981818
TCTATAC134600.014.70447353
ATCGGAG32300.014.5940637
TGCTCGG51200.014.5875865
GCTCCGA30400.014.5509826
CTACACT116300.014.1518874
Produced by FastQC (version 0.11.5)