FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_30893.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_30893.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19426433
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA71050.068.075756
TCGGAGA78800.065.5275048
CGGAGAT82000.064.299619
CTCGGAG88450.055.5884977
GGCTCGG77900.052.4996835
GGGCTCG68200.048.584244
CGTGGGC77650.028.1907141
TGGGCTC214150.016.0801433
TATACTG205800.015.2565945
GTGGGCT148350.015.1479112
TACACCG40750.015.1253815
CTAGGAC80550.014.3102093
TACACTG156950.013.9527785
CCTACAC93300.013.9086753
GTGTAGG94950.013.8173311
CTACACT119350.013.7900884
TACACAG195150.013.673965
GTGTTAG138300.013.6267341
CTTACAC114150.012.8289133
GTATAGA140400.012.7001421
Produced by FastQC (version 0.11.5)