FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_30891.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_30891.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14452372
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG166390.11512989009693356No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA84100.046.474596
CGGAGAT102700.041.524119
CTCGGAG98350.040.1114547
TCGGAGA108600.038.130788
GGCTCGG90700.036.7744565
GGGCTCG78450.034.011444
TGGGCTC93400.029.0301863
GTGGGCT93000.019.0185032
TATACTG105250.015.9142285
TATACCG51900.015.3681325
CTATACT98800.015.3376754
GTATAGG67700.015.0961211
CGTGGGC111650.015.0613581
GTATAGA111650.014.93151951
CCTATAC65350.014.8656643
TACACAG87000.014.5852735
GTGTAGG79200.014.5514691
TACACCG79150.013.7415625
TAGACCG34550.013.6414675
TGGACCG49350.013.5175015
Produced by FastQC (version 0.11.5)