FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_30886.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_30886.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19510451
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA72900.079.676196
TCGGAGA82350.074.062118
CGGAGAT84350.072.9106759
CTCGGAG89450.064.533347
GGCTCGG80500.062.33275
GGGCTCG66550.059.9204034
CGTGGGC74050.033.4759671
TGGGCTC221250.018.7096713
GTGGGCT151450.017.0392552
GATCGGA20500.016.9789566
TATACTG223950.016.7396075
TGGCTCG50750.016.5722714
TACACTG162800.016.4798435
CTACACT124400.014.8038064
GTGTAGG91400.014.5123231
CTATACT141900.014.2554824
GTGTTAG144700.014.1257861
GTATTAG138600.014.0676361
TCTATAC136650.013.740533
CTAGGAC83150.013.6011513
Produced by FastQC (version 0.11.5)