FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n02_30786.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n02_30786.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15230211
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCGGA79950.032.017066
CTCGGAG85000.029.348427
CGGAGAT103650.026.238739
GGCTCGG85600.025.4128155
TGGGCTC73250.024.545443
GGGCTCG72850.024.4835134
TCGGAGA109650.024.3386928
TATACTG118400.016.7192295
TACACTG77400.015.3641355
CTATACT106950.015.0501334
CTACACT82750.014.9829144
TACACCG90950.014.5102185
GTATAGG87650.014.1408461
GTATAGA129300.014.07041
TATACCG67000.013.8528915
TCTATAC129050.013.7075123
ACCGTGC91700.012.9697788
GTGTAGG88850.012.8893451
TCTACAC121350.012.8447453
GTCCTAG26600.012.8070011
Produced by FastQC (version 0.11.5)