FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38284075
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26525796.928674651274715No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG137750.014.6196975
TATACTG182000.013.7726235
TAGACAG200350.012.0834595
GTGTAGA114100.012.0817951
CTATACT128250.011.7487544
CTACACT113550.011.6974864
TACACAG176550.011.4876585
CTAGACT86700.011.2017664
TCTATAC128300.011.1875063
GTATAGG81250.011.1645221
GCTAGAC70650.011.1184543
GTCCTAG69650.011.0754771
TATACAG183950.010.9478855
CTAGCAC93100.010.8918713
CTTACAC119200.010.7236013
ACAGTGC120550.010.7171828
CTAGGAC66150.010.687333
TACACCG36100.010.6823665
GTATAGA131750.010.6801021
TAGACTG101900.010.652475
Produced by FastQC (version 0.11.5)