FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_Neg.2.21_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_Neg.2.21_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences67
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TAAGAATTCTCCTGCATCATATAATGGGCTTAGAAATTGAGAATAATTTT11.4925373134328357No Hit
CATGTATTCAGTTGTAAGAAATGTGGTCTTCAAAATAATTAAGCCACGTA11.4925373134328357No Hit
GTTGGAACCATCTCTACTACTGTGGGTCTTGTCAAAATCAGCAAGGCGTC11.4925373134328357No Hit
AAATAACAGTCTTGTAACTGTAGTAATCATAAGGAAATTTTCTCCTTGAA11.4925373134328357No Hit
GTGATACAGCTAGAAAGAGGAAGAGCCAGCCTTCAAACATAGGTGGTGAG11.4925373134328357No Hit
TTTCTATTCTGCTCATAAATGACCTGGCAAATTTTGATTTTAGAGATTGT11.4925373134328357No Hit
CACCGTGCGCGAGCCGAAGCAGGGCGAGGCATTGCCTCACCTGGGAAGTG11.4925373134328357No Hit
CTATTGACGTGGGGTCCTTCTACCTAAAGACCCCCCCGTCACATAAGCAC11.4925373134328357No Hit
GTATTTGTTTGTCTTTTTGTATACACTCATACACCCACAATGAATTTTAA11.4925373134328357No Hit
TGGAATGACCATCAAGTTAAATGGCTGGAAAACTTAAGCTGAGAATGGTG11.4925373134328357No Hit
GTCCCTGGGGGAAAAGTAGATTGGGGGGCAAAGGCGAATGGTATGGTAGA11.4925373134328357No Hit
CCACTCTGAGGTCTTAGGCACTGGAAATGGGGCCTTAGGCATCCGTGGGA11.4925373134328357No Hit
GGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAG11.4925373134328357No Hit
AATTATATAACAACTGTATAATCTTCCTATCATCCCGATCACAGAGAGTT11.4925373134328357No Hit
GTATATAGTTCAATTAAACTAATTATTTTCTCATCCTATTTTCAGATAAG11.4925373134328357No Hit
GAATTGGATGATGCAGTTAGCAACTTTTCATGACCTCTGCTGTCCCACAT11.4925373134328357No Hit
GTCCCGCAGTCTGCTCTTCCTCCGCACAGCCAGCAGCTGCAGGGTCAGGT11.4925373134328357No Hit
TCTTTGCATCCTTAAAACCTAATATAACAGGTGATAAGGAAGTGCTCCTC11.4925373134328357No Hit
ACCTAATAGTATACATAATAGTTGTGTTGGTTGAGAGGTCCTAGAATCAA11.4925373134328357No Hit
CCTTCTGCATTTTTCATACAAATTGGAAAAAAAAAAAAACAAGAGTAAAA11.4925373134328357No Hit
AGATAATACTGTTACAAATTGATATTTTTTGAGGAATAACATTTGAAATA11.4925373134328357No Hit
ATTCTGCAGGAAAGCTATGTTTTGTTGTAAGACACATTAGCTCTTACAAA11.4925373134328357No Hit
TCACAGCACTATCTATGGTGCAAATGAAATATTCAAGTATACAGTCTCTT11.4925373134328357No Hit
TTAGGAAACTGCTACTAAATTGAGTAATAAAATGGTACACAGATATTTAA11.4925373134328357No Hit
CTATACCACTATTTCTCTTCAACTAGTCCTTACTTTTATAAACAAAAAAG11.4925373134328357No Hit
GTGTATATTCACTGCGTATATGTTCATCTGTCCCTTCCTATGTACCAAGC11.4925373134328357No Hit
CCCTACAGAAAATGAGACTGAGAAATTCCAACATAGCACAAAGAATATCA11.4925373134328357No Hit
GTTCATAAGGGAACAGGGGGAAGTGGTTGCCTTCAGAAACCATAATACAT11.4925373134328357No Hit
GCTGGAGGCTCCTGGGTCCTCCAGGACAGCCGCTGAAAGTTTGGCCCTGC11.4925373134328357No Hit
GCCTCAGCCTCTGAGTAGCTGAGACTACAGGTGTGTCCCACCACACACAG11.4925373134328357No Hit
GAATGCTTCTGTCTAGATTTTATATGAAGATATCCCGTGTCCAACGAAAT11.4925373134328357No Hit
AAATGGAACGGAAAGGAATGGAATGGAATGGAATGGAATGGAATGGAATG11.4925373134328357No Hit
GCATTACAGACAAAACAATATTTGTTTAACACATATAGCAAAATATCATT11.4925373134328357No Hit
GTATCCAGAAGCACATCAAAAAGCTTATCCACCATGATCAAGTCGGCTTC11.4925373134328357No Hit
GGCCAGACAGGTCTTGAACTCCTGAACTCATGATAAACCTGCTTTGGCCT11.4925373134328357No Hit
GATGATTAATTATTTATGCTTTTGTATTATGATACCCCAATATAATCCAC11.4925373134328357No Hit
GTTCTAAGTAACACATTTTTGATCCAGTTTGATTCTAGAAGCATTTTAAT11.4925373134328357No Hit
GTAAAGAATTGCTTCCCCTTTCTAGGTCTTTTCCTGATCCTGAAGAAATT11.4925373134328357No Hit
CCGCAGCTGGCTAACAGAACCAGTTGTGAATACGCAGTGAGTTAGAATGA11.4925373134328357No Hit
CTCTCCTTCCGCATTTGGCCTGTTGTCATAGCACACATCATGTTACCTCT11.4925373134328357No Hit
CTTGTAAGTTGGATTCCTAGGTATTTTATTCTCTTTGAAGCAATTGTGAA11.4925373134328357No Hit
ATGCTGCATAGAGAAGTAAAGAAGCTTTGATGGAAATGTTCATCAGAGAA11.4925373134328357No Hit
CAATATCATTTTTAGCGCAGATAGTTTTTAATTTTAGAAATGTACCATAA11.4925373134328357No Hit
CCACAGCACTCAGAGAGTGATTTGCTATGAACCAACAGCAGGTAGCTATA11.4925373134328357No Hit
GATATACACTTCAAGATGTGCATATCACAAATCCGTTATACTAATTTTTA11.4925373134328357No Hit
GGCACCATCACTCACCCTCATTTTTTATTTTTATTTTTTTTTGAGATGGA11.4925373134328357No Hit
CGCGCGCCCCCTGAGCTTGTAAAGCAAGTGCAAGGGTCACCGACACAGAA11.4925373134328357No Hit
CTATTTAAAACATAGCCCCATTTAGCCACTCAGGTTGTATTTTGAAAGAT11.4925373134328357No Hit
GCCGGGGGCTCAGGCCCCGAACTCGATATCCTTAACTTAATCCAGCCAGT11.4925373134328357No Hit
GGCGCAGAGGCCTGACAGTGGTCTTCTCACAGCTTCGCTAGGTGGTGCCC11.4925373134328357No Hit
TCCTACAGCAGCGTGACAGCAAACTGGGCGTTATTCAACTCTACAGATGA11.4925373134328357No Hit
GTGCTTGACATATAATAAATACTTAGTAAAATGTGATTGCTATTGCATTA11.4925373134328357No Hit
TAGCTACTGACACCTGGCTTCTAGAAATTCAAGGTTCTGTGATTCTCATT11.4925373134328357No Hit
GTTCTCAACCCTCCTAATTTGTCTTGCTCAATATACCAGGTCTTTCCATG11.4925373134328357No Hit
GTGGTTGTCAATAGGAATTTATAAAAACTCACCCTATTATCTAAAAATAG11.4925373134328357No Hit
GAGCTCTACAAGCTGATTTACGGGCTGTCCGTGGAGTAGAAGTGAATGGT11.4925373134328357No Hit
CGGTAGCGCCCTTTAGCCCATATATCCTCCCCTTTAACCGACTATAATCA11.4925373134328357No Hit
GAAGATTGTAATGTAAACAGCAATATATAAATCAGACAACCAAAAAGCTG11.4925373134328357No Hit
GTGGTGGCTTATGCATGTAATCACAGACGTTTGGACGGCCGAGGCGGGCA11.4925373134328357No Hit
ATCAAAGAGCTTAAAATCATAATTTTCATTCAATAGCAAGTCGAGTTGTC11.4925373134328357No Hit
CTAAAGGAGAGAGATGCATGTGCACACATCACACATCCTTGTTTAACCTA11.4925373134328357No Hit
GCCACGAATGTCAAAATCCCTTCTAATACCAATAAAATTTCAGAACTCAA11.4925373134328357No Hit
CCTAAAACTCAGTGTGATAAGTATGATTCCGAATTGCTGTCCCATACCTC11.4925373134328357No Hit
GTATATGATTGATCTGATAGGTCCTTATAATAAGAGACAACAGAGAATTT11.4925373134328357No Hit
GGTGAGGCAAGCAGGCAGCCAGTCTCCAGAGGCTGAAGAATTCAGGGAAG11.4925373134328357No Hit
AACCCCGTCTCTACTAAAAATAGAAAAAGTTCGACGGGCTCTGTCTCTTC11.4925373134328357No Hit
CAGCAGTACCACGAGATTTAATAAAAAGTATAAACTTACAAAAGCAAAGT11.4925373134328357No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGACA50.0145.06
AGCTACT50.0145.02
TAGCTAC50.0145.01
TGACACC50.0145.08
CTGACAC50.0145.07
GCTACTG50.0145.03
GACACCT50.0145.09
TGCTTAT50.0145.0145
TACTGAC50.0145.05
CTACTGA50.0145.04
Produced by FastQC (version 0.11.5)