FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_MG_30908.TR4_batch_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_MG_30908.TR4_batch_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11341662
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG137100.018.2428635
TACACTG96500.016.6777345
ATGCCGG53700.014.85424145
CTATACT83000.014.4117674
CTACACT71050.014.3868734
GTACTAG36350.014.162941
GTATTAG80200.014.1042391
GTATAGA84150.014.04536151
GTCCTAG51500.013.5164761
TCTATAC82300.013.4773023
TATACAG108850.013.4534695
ATACTGT176600.013.3825326
CGTACAC15550.013.0538633
GTCTAAG52250.012.9061331
TAGTACT64200.012.8730424
GTGTAGA69450.012.7375831
CTAGCCT75850.012.5206574
TGCACTG102100.012.3547755
TAGACAG145100.012.3407325
GTGTAGG53550.012.3220041
Produced by FastQC (version 0.11.5)