FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_9424.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_9424.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14193000
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG108250.017.211265
TATACTG155950.016.7349535
TATACCG26950.015.3328715
CCTACAC75800.014.8242053
CTATACT104200.014.2624314
TCTATAC103400.014.2326073
CTTACAC87100.013.9829913
GTATAGA106750.013.9248751
GTATAGG55300.013.899081
TACACCG37750.013.826845
GTACTAG44100.013.4828161
GTCTAGG57650.013.4582871
CCTATAC59400.013.4250273
CTACACT88100.013.33050254
CTAGGAC53000.013.2679813
GTAGGAC45050.013.1955653
TACACAG139350.013.1619725
GTGTAGA95200.013.0246031
CTACACC81600.012.97094
GTATTAG107300.012.772251
Produced by FastQC (version 0.11.5)