FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_8336.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_8336.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15060836
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG171000.014.8382265
TACACTG124750.014.7605735
TACACCG32750.014.1670275
GTATAGA124750.014.0675191
TATACCG26300.014.058035
GTATAGG66500.013.9582531
GTGTAGG71500.013.8949591
CTAGGAC57050.013.7239653
GTACTAG47800.013.6539191
ATACTGT213650.013.4709336
TAGGACT87150.013.3927134
CTACACT96900.013.2421834
GTATAAG115700.013.2248831
CTATACT118300.013.17544
GTATTAG120450.013.1247921
GTGTAAG102900.012.8262221
TAGACTG95600.012.6639345
TCTATAC118550.012.65844253
CTTACAC98350.012.531013
GTCCTAC51100.012.4883341
Produced by FastQC (version 0.11.5)