FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_8230.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_8230.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15125718
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG58550.017.834787145
TACACTG131650.016.9606725
TATACTG189500.016.5650165
AGACCGC58650.014.960626145
CTACACT93750.014.8472014
TACACAG165950.014.3287995
CTCCGAC24150.014.112797145
GTATAGG60350.014.0589361
GTATAGA115050.013.8669171
GTCCTAG71150.013.7595021
TCTATAC112350.013.5507623
GTGTAGG72500.013.503291
CCTACAC72550.013.3901343
GTATTAG104100.013.3750171
CTTACAC96150.013.3457043
GTGCTAG82500.013.2729311
CTAGTAC47600.013.2504243
GTGTTAG114400.013.1216421
CTATACT119500.013.1038974
GTATAAG104700.013.0905831
Produced by FastQC (version 0.11.5)