FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_8230.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_8230.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14853912
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG180400.016.4361155
TAGATCG40800.015.6403885145
GTATTAG107300.015.4762821
TACACTG129650.015.0974525
ATGCCGG58250.015.063104145
CTACACT97750.014.6845574
CCTACAC69550.014.0717813
GTATAGG59000.013.8885621
GTGTAGG68050.013.7465091
CTAGGAC66750.013.5759783
GTATAGA107100.013.4739351
TAGACAG197050.013.281385
CTTACAC97600.013.2215583
CTATACT113650.013.2042384
CACGAGC124300.013.184459145
GTGTAAC62200.013.1740381
GTAAGAC78200.013.0714813
TCTCCGC103900.012.981427145
ATACTGT227450.012.9724176
TCTGTCG50500.012.9199898
Produced by FastQC (version 0.11.5)