FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_8230.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_8230.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18034186
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG181900.016.2605365
TACACTG123800.015.9863545
TATACCG42200.014.9456295
CTATACT134150.014.2666064
CTACACT109700.014.2082064
GTATAGA141200.014.1742421
TCTATAC134200.013.721093
GTACTAG61250.013.4965941
CCTACAC87550.013.4142473
GTATAAG129200.013.4140781
GTCCTAG60550.013.4131041
TACACAG155800.013.2612615
TAGACTG100750.013.1678075
GTATTAG161400.013.0741931
CCTATAC80600.013.0418863
CTACACC116250.012.8464074
CTAGTAC54000.012.7537313
TCTACAC120450.012.6993963
TACACCG55550.012.65887455
GTATAGG82650.012.6341221
Produced by FastQC (version 0.11.5)