FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_7920.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_7920.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13875352
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG185200.017.7715195
CTATACT110900.016.7347054
GTATAGG56500.015.5294521
TCTATAC109850.015.5088853
TACACCG14550.015.4457265
CCTATAC59350.014.6579513
ATACTGT218450.014.6019316
TACACTG129700.014.3090115
GTATAGA108100.014.2880541
TACACAG156500.014.0821485
CCTACAC68800.013.8037053
CTTACAC90450.013.5453723
GTGTAGG66400.013.3232751
CTACACT93250.013.2162954
GTGTAAG98750.012.9239351
GGATCGT19850.012.782548
GTATTAG101700.012.7629571
ACTGATC94300.012.7616278
TAGACTG103700.012.7233735
GTATAAG97200.012.607811
Produced by FastQC (version 0.11.5)