FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_7651.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_7651.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14093173
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG188450.016.8880275
TACACTG132150.016.8415375
GTGTAGG71150.016.714861
CTACACT95300.015.21414854
AGACGGC58200.014.952177145
ACGGACG11650.014.9393425145
GTGTAAG99400.014.8825591
ATACTGT229300.014.8595066
CTATACT111900.014.7711854
GGATCGT20600.014.076788
TATACCG11900.014.011725
CTTACAC97600.013.8900533
CTGTCGC44450.013.3737339
GTATTAG102800.013.3322221
GTGCTAG79700.013.1929991
TCTATAC105200.013.1622373
GTCTCGC43250.013.0780291
GTATAGA110500.012.9938161
TAGACAG210100.012.9049445
GATCGTT23600.012.9017289
Produced by FastQC (version 0.11.5)