FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_7651.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_7651.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12839717
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG149550.015.5607795
TACACTG109650.014.8760155
CTCCGAC19400.014.577469145
CTACACT85500.014.414424
GTGTAAG84350.013.670161
CTATACT101650.013.62198454
GTATAGG51450.013.5315461
GTGTAGG60150.013.5034251
GTATTAG92400.013.4995111
GTATAAG91250.013.3517431
GTAGGAC38200.012.7152773
GTAAGAC70250.012.5900633
CTAACAC98100.012.5630273
CTGTCGC45650.012.5458189
CTAGTAC40500.012.5301833
TAGGACA83350.012.5248144
ATACTGT192200.012.3340886
CCTATAC52550.012.2781143
TCTCCGC72850.012.2431965145
TCTACAC87600.012.1654343
Produced by FastQC (version 0.11.5)