FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_6852.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_6852.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17066207
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGG83550.022.393408145
TATACTG191350.016.4046275
TACACTG147150.015.6172995
CTCCGAC30750.015.329046145
CTATACT127250.014.8692694
GTATAGA119200.014.4786651
TCTCCGC165200.014.354386145
CGAGACA70400.014.318221145
CTACACT107100.014.2823594
GTATTAG125650.014.1971261
CCTACAC75850.014.0497943
GTGTAGG76500.014.0290441
GTCCTAG76900.013.8617741
TACACAG181950.013.8256115
GTATACG18100.013.6216021
GTGTAGA103350.013.4014171
GTGTAAG105150.013.3099341
TAGACTG116050.013.3058135
CTAACAC128850.013.2218333
GTATAGG68050.013.1070451
Produced by FastQC (version 0.11.5)