FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_6852.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_6852.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21757847
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG261150.016.6005845
TACACTG190450.015.0358635
CTATACT165050.014.6704414
GTATAGA160000.014.2763961
CTCCGAC36150.014.242755145
CTACACT138800.013.8410384
CCTACAC99300.013.7252673
TCTATAC156350.013.53933053
GTGTAGG97750.013.5015191
CTTACAC136600.013.21480753
TAGACTG143750.012.9105395
GTATTAG157600.012.8373681
ATGCCGG212550.012.725994145
ATACTGT340400.012.6079186
GTGTAAG138750.012.5954041
ATAGGAC89800.012.5131763
TACACAG236450.012.3866625
GTGTAGA137900.012.3575281
TAGGACA135600.012.3499324
CACGAGC116350.012.340786145
Produced by FastQC (version 0.11.5)