FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_6331.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_6331.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17774129
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC31300.017.376804145
TCTCCGC119700.017.084723145
TACACTG158950.015.7348875
CACGAGC83150.015.524253145
GTATAGG69550.015.222311
ATGCCGG89500.015.071027145
TATACTG198850.014.72857955
GTATTAG129150.014.2614651
GTGTAGG77250.013.7050051
GTCCTAG78100.013.6486961
TAGACTG125850.013.4217075
CTATACT134800.013.3372684
GTATAAG122300.013.2814781
CTACACT118750.013.0032264
GTGTAAG120700.012.9168331
CGTACAC19700.012.8798073
CTAGACT99000.012.8146864
CCTACAC81500.012.6310163
TATACAG171650.012.4590035
TACACCG22900.012.3462265
Produced by FastQC (version 0.11.5)