FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_4329.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_4329.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17886525
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG177150.015.6736865
TACACTG126450.015.5368875
TACACCG71200.015.2730155
CCTACAC100100.015.2090183
CTACACT108200.015.0084814
CTATACT130800.014.41058354
TAGACTG93500.014.3441115
GTATAAG144150.014.034961
TATACCG54400.013.3264555
GTATAGA142300.013.3001711
GTATAGG85400.012.9913761
GTGTAGA136350.012.923281
TACACAG146800.012.8398765
TAGACAG149650.012.8375665
TATACAG175250.012.8238135
GTGTAGG99000.012.7448781
GTCCTAG52350.012.7436181
TCTACAC134200.012.6409393
TCTATAC147200.012.4603063
CTTACAC108900.012.3822913
Produced by FastQC (version 0.11.5)