FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_341.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_341.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14396135
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG169750.016.6134995
TACACTG128300.016.1042145
GTGTAGG65250.014.4479531
GTGTAAG97200.013.8768261
CTATACT109200.013.8089934
CTACACT96850.013.4739274
GTATTAG106000.013.2036781
CACGAGC47950.013.156792145
CTAGGAC61800.013.1388363
TAGGACT85650.012.9504644
CTTACAC93600.012.7801343
ATGCCGG50750.012.716667145
ATACTGT215800.012.5306666
CTAGTAC46550.012.4594143
GTATAGG58800.012.4562561
GTATAGA107200.012.3117591
GTCCTAG66750.012.1677491
TAAGACT124350.012.0682944
GTAAGAC79350.012.0601963
TCTATAC105050.012.0082713
Produced by FastQC (version 0.11.5)