FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_31013.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_31013.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16408104
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG144900.017.0606255
TATACTG189900.016.8353675
CTACACT108550.016.1619784
CTCCGAC34450.015.997853145
ATGCCGG136600.015.607522145
GTATAGG63300.014.6636571
CCTACAC79600.014.57202153
GTCTCGC53250.014.0266391
GTGTAGG77600.013.9238881
TCTGTCG55750.013.9137828
CTTACAC108200.013.8023453
GTATTAG114050.013.7975021
TACACAG180600.013.7283175
TAGACTG114700.013.6520955
GTATACG16650.013.5015441
CTATACT121450.013.4902474
CTGTCGC61750.013.1488379
TCTCCGC141750.013.045312145
ATACTGT255850.012.920776
TATACAG159100.012.8039835
Produced by FastQC (version 0.11.5)