FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_30981.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_30981.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24041603
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACG39700.019.362164145
CTCCGAC44000.017.964386145
ATGCCGG157750.016.962723145
TATACTG268550.016.2780865
TACACTG209850.015.6494975
GTATAGG94500.015.1170651
CGTACAC24300.014.6185893
GTGTACG22000.014.5031761
GTATAGA163850.014.11813451
AGACGGC251350.013.906142145
CTACACT149150.013.9012534
GTGTAAG155150.013.507631
CTATACT173300.013.4281944
TCTCCGC218300.013.353983145
TCTATAC167250.013.3505843
CACGAGC163350.013.273657145
TACACAG265750.013.2578815
GTCTTAC121550.013.2443641
GTGTAGG106050.013.0603791
TAGACAG319750.012.6966355
Produced by FastQC (version 0.11.5)