FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_30981.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_30981.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15609693
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCCGAC34050.016.185997145
GTGTAGG70800.015.5681351
TATACTG155800.015.5413395
GTATTAG105400.015.1359041
TACACTG129950.014.6161995
CTACACT97200.014.1708774
GTGTAAG95650.013.6462651
CTATACT104950.013.5388894
GTATAGA103000.013.5173091
CTAGGAC66700.013.4773723
CTTACAC95900.013.2290563
TATACAG139850.013.1668125
TAATACT168100.013.1103794
GTATAGG57050.013.0920561
CCTACAC72850.013.0362033
TACACAG163100.012.93446355
CTAACAC120950.012.8867083
GTCCTAG68800.012.8587191
TCTATAC104200.012.801463
CCTATAC59850.012.718463
Produced by FastQC (version 0.11.5)