FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_30979.TR2_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_30979.TR2_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20096251
Sequences flagged as poor quality0
Sequence length151
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACTG178400.017.2297765
TATACTG224000.015.923035
TAGCGCG31900.015.912727145
GTATAGG80650.015.0156061
CTATACT146950.014.5039534
TATACCG20300.014.2847495
TCTGTCG68550.014.2769358
GTGTAGG94750.014.235231
GTATAGA145300.014.1737081
GTGTAAG131050.014.054891
CTACACT134900.014.0261094
TCTATAC142250.013.9130823
CTTACAC129900.013.4499563
TACACAG220100.013.273755
ATACTGT290050.013.2718146
CTGTCGC74350.013.2607049
TCTCCGC110800.013.155081145
GTACTAG67700.013.0677821
GTGTATA255350.012.8645051
GTATAAG138200.012.8030231
Produced by FastQC (version 0.11.5)