FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_30907.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_30907.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14672278
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG164250.015.3158355
TACACTG123150.015.1291785
GTATAGG61000.014.2639741
CCTACAC73700.014.2632053
CTACACT92650.014.0845744
TACACAG157800.014.01230055
CTTACAC91400.013.3253823
GTATAGA108600.013.2865541
GTACTAG47550.013.2665461
GTATTAG107100.013.1341311
TCTATAC106600.012.7854873
CTAACAC105350.012.6619293
TAGACAG178350.012.5603575
GTGTAGA101250.012.4607271
GTGTAGG70500.012.4447281
GTATAAG98350.012.3120741
CTATACT106600.012.3094314
GTGTTAG109600.012.3052811
ATACTGT210150.012.2466016
TAGACTG93000.012.2386445
Produced by FastQC (version 0.11.5)