FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_30905.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_30905.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17883448
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG187900.015.3941765
TACACTG134700.014.80046755
CTACACT105500.014.2929284
TACACCG54300.013.6179165
GTATAGG71600.013.3687331
CTATACT122300.013.2186994
CCTACAC87900.012.9486213
TATACCG38200.012.9049375
GTATAGA128600.012.7437371
CCTATAC72150.012.5599073
TACACAG165500.012.5279025
GTGTAGG86250.012.5272711
GTCTAAG78150.012.4338361
GTCCTAG65900.012.324291
CTTACAC103950.012.2744183
ATACTGT244050.012.14946
TAGTACT92600.012.1347564
TAGACTG102350.012.1120755
GTGTAGA113250.012.037851
TCTATAC121950.012.0083213
Produced by FastQC (version 0.11.5)