FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_30900.TR4_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_30900.TR4_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18558185
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG215900.017.0913315
TACACTG154100.017.030085
CTATACT136950.014.8749334
ATGCCGG121650.014.843293145
CTACACT116750.014.8406114
CCTACAC88300.014.7783163
GTATAGA138100.014.4400261
TACACCG33950.014.3069095
TCTATAC138250.014.0010053
GACCGGC114850.013.827897145
GTCCTAC55700.013.8000171
CTAGGAC80000.013.2304923
GTGTAGG87650.013.0717611
TACACAG185550.012.9714315
GTGTAGA126050.012.9440031
ATACTGT281450.012.6986186
GTGTTAG134550.012.5574421
TATACAG184750.012.5567225
CCTATAC74150.012.5144543
GTATAGG75450.012.4943331
Produced by FastQC (version 0.11.5)