FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_30893.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_30893.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19426433
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGCG52650.018.731632145
TATACTG212850.015.3949015
CTACACT119800.014.8864244
TACACTG156500.014.869725
GTATAGG74750.014.6489491
CTATACT142400.014.4584084
GTGTAGG89950.014.3502511
CCTATAC76850.013.9615043
GTATAGA135500.013.4865831
GTGTAAG115600.013.2989921
TCTATAC136550.013.2728033
TAGACTG125100.013.1546925
TACACCG40250.013.1482855
GTGTAGA129600.012.8695541
CTAACAC139050.012.8256233
CCTACAC90150.012.7059083
GTATTAG143300.012.5500711
GTCCTAG85450.012.4751551
CTAGTAC63950.012.2432733
TCTCCGC175350.012.241117145
Produced by FastQC (version 0.11.5)