FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_30891.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_30891.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14452372
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACTG107850.017.0062125
TACACTG72300.016.845285
CTATACT97600.015.1526164
GTATAAG101850.014.8799231
GTATAGG65000.014.7257021
GTCCTAG34300.014.5871481
TACACCG82350.014.2612835
TATACCG49250.013.9837815
TCTATAC106350.013.8378553
GGACCGT38350.013.6105096
CCTACAC74000.013.2255053
CTACACT69350.013.0668474
TAGGGTG43450.013.0140375
GTACTGG83800.012.979641
TAGACAG76600.012.871155
GTATTAG120700.012.6762441
TAGACTG53400.012.6255095
GTGTAAG75500.012.5817131
TTATACT170600.012.5782414
GTGTAGA101350.012.4491851
Produced by FastQC (version 0.11.5)