FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_30886.TR1_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_30886.TR1_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19510451
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGACG31050.017.048832145
TATACTG224850.015.7341135
GTATAGA135800.015.0050181
TACACTG163400.014.463755
TATACCG23300.014.0014155
CTATACT136650.013.6344874
CTACACT122750.013.3475734
ATGCCGG104450.013.260449145
TACACCG29800.013.1368995
TACACAG202900.012.9699985
TCTCCGC137250.012.944553145
GTATTAG136450.012.8609131
GTGTAAG127050.012.8421541
GTCCTAG86600.012.81160451
TCTACAC124950.012.7645383
CACGAGC103600.012.739283145
GTGTAGG94250.012.6180571
CTAGTAC61050.012.5874823
AGACGGC160800.012.4918585145
CCTATAC75550.012.47462753
Produced by FastQC (version 0.11.5)