FastQCFastQC Report
Thu 28 Mar 2019
H53K5BGXB_n01_30786.TR3_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K5BGXB_n01_30786.TR3_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15230211
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACACCG90900.016.509035
TATACCG67800.015.9320495
TATACTG119150.015.8804025
TATACAG138350.015.6677675
TACACTG71950.015.0131045
CTATACT105850.014.6567594
CTACACT75150.014.2773564
TACACAG91300.013.8163575
CTAGACT32700.013.745454
TCTACAC114750.013.7096053
GTATAGA133400.013.6435391
GTATAAG119950.013.4807411
GTGTAGG91450.013.4002271
CTACACC139700.013.3886994
TCTATAC127250.013.3883793
TAGACTG55700.013.2757795
ACCGTGC91300.013.1810798
TAGGACA65950.012.8613374
CTAGGAC24000.012.6869133
TCTAGAC35500.012.6613863
Produced by FastQC (version 0.11.5)