FastQCFastQC Report
Wed 27 Apr 2022
H53K3DRX2_3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53K3DRX2_3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1016132919
Sequences flagged as poor quality0
Sequence length10
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAACAGGAA34419361333.87289266631859No Hit
TCCTCGAATC32104647431.59492897011439No Hit
TTTAGACCAT30852216330.36238244339371No Hit
TGATCTCGGT142269081.4001030508883652No Hit
AGATCTCGGT53361770.5251455690709691Illumina Single End PCR Primer 1 (100% over 10bp)
GGGGGGGGGG17835130.1755196556130862No Hit
TAAACAAAAA11655810.11470753266679672No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)