FastQCFastQC Report
Wed 21 Apr 2021
H53FKDRXY_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53FKDRXY_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090136733
Sequences flagged as poor quality0
Sequence length8
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGAGGCC12783983911.726954530574469No Hit
AACTGGCG11136241410.215453770972049No Hit
TTGGCATA1071931609.833001380020463No Hit
GGTAACGC1069175769.807721615413174No Hit
CCACTTAT1042177479.560061948669333No Hit
ATCTTTAG997318149.148560082508475No Hit
GGTCAATA887323728.139563534916679No Hit
TCAGCCGT628910195.7690945636633275No Hit
ATCTCTGT566235785.194172096574971No Hit
TAGCTCTA563727955.171167367681022No Hit
CCTAGACC549878115.044120552536229No Hit
GGAGAGAG516172134.734930164031084No Hit
GGGGGGGG223002872.0456412782854096No Hit
GGAGCGAG18009410.1652032213467299No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.11.9)