FastQCFastQC Report
Mon 28 Jun 2021
H53C5BGXJ_n01_3QG3464.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53C5BGXJ_n01_3QG3464.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1524510
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCTCGTAT270031.771257649999016TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATGTCGTAT236781.5531547841601565TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATGGCGTAT51380.3370263232120485TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATCGCGTAT31020.20347521498711063TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATGTCGGAT18900.12397426058208869TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATGTCGTTT18690.12259676879784323TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGAGAGAAATGGCGGAT17090.11210159329883045TruSeq Adapter, Index 2 (97% over 36bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)