FastQCFastQC Report
Mon 28 Jun 2021
H53C5BGXJ_n01_3QG3391.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53C5BGXJ_n01_3QG3391.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444626
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTGACCTATCTCGTAT184094.140333673694296TruSeq Adapter, Index 13 (97% over 38bp)
CTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCT7710.17340416439884307No Hit
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC7110.15990967689698757No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG5990.134719966893524No Hit
CCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCC5640.12684818251744162No Hit
GCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGC5580.1254987337672561No Hit
CTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCT5280.11875149001632833No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)