FastQCFastQC Report
Mon 28 Jun 2021
H53C5BGXJ_n01_3QG3342.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53C5BGXJ_n01_3QG3342.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9369821
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGGGGTT257250.2745516696637001TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGGGGAT189600.20235178452181743TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGGGGGT135410.14451716847098786TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGCGTAT131800.1406643734175925TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGGGTAT128760.13741991442525955TruSeq Adapter, Index 22 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGGGTTT118120.12606430795209428TruSeq Adapter, Index 22 (97% over 38bp)
CCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCC112470.12003431015384393No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCTCGTAT111390.11888167340656774TruSeq Adapter, Index 22 (97% over 38bp)
CTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCT108910.11623487791282246No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTACAGCATCGCGGAT108430.11572259491403304TruSeq Adapter, Index 22 (97% over 38bp)
GCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGCCTAAGC105180.11225401210972974No Hit
GGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGGCTTAGG96280.10275543150717607No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)