FastQCFastQC Report
Mon 28 Jun 2021
H53C5BGXJ_n01_3QG3255.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53C5BGXJ_n01_3QG3255.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5398116
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGAATCTCGTAT688121.274741039281112TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGAATCGCGTAT127610.236397291203079TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGAATCTCGGAT99420.18417536785056118TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGAATCTCGTTT85900.1591295926208329TruSeq Adapter, Index 2 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAATGCGAATCGCGGAT76060.14090101064890048TruSeq Adapter, Index 2 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)