FastQCFastQC Report
Mon 28 Jun 2021
H53C5BGXJ_n01_3QG3223.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53C5BGXJ_n01_3QG3223.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences901113
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGTAT95181.0562493272208924TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGGAT32530.3609980102384496TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCGCGGAT28670.31816209509795107TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCGCGGTT28430.31549872213584756TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGTTT28110.31194755818637615TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCGCGTAT24320.26988846015982454TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCTCGGTT20510.227607414386431TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCGGGGTT19560.21706489641143784TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCGCGTTT14420.1600243254730539TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCGGGGGT10770.119518861674396TruSeq Adapter, Index 19 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACCGAATCGGGGAT9880.10964218693992873TruSeq Adapter, Index 19 (97% over 37bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)