FastQCFastQC Report
Mon 28 Jun 2021
H53C5BGXJ_n01_3QG3108.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH53C5BGXJ_n01_3QG3108.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335176
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCTCGTAT232251.7394710510075078TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATGTCGTAT135821.0172441685590514TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATGGCGTAT103910.7782494592473201TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCGCGTAT91350.6841794639807786TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATGGCGGAT65840.4931185102188775TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCGCGGAT38090.2852807420145359TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATGGCGGTT34730.2601155203508751TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATGGCGTTT34320.2570447641359641TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATGTCGTTT27450.2055908734129433TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATGTCGGAT27000.20222053122584588TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCTCGGAT25240.18903874844964258TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATGGGGGTT23600.17675572358999864TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCTCGTTT23530.17623144813867236TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATGGGGGAT21450.16065297758497757TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATCGCGTTT18600.13930747706669383TruSeq Adapter, Index 4 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACCGACATGGGGTAT15650.117213011617944TruSeq Adapter, Index 4 (97% over 38bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)