FastQCFastQC Report
Tue 15 Jun 2021
H533TBGXJ_n02_cDNA_C3_210615.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH533TBGXJ_n02_cDNA_C3_210615.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5984961
Sequences flagged as poor quality0
Sequence length57
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT853881.4267093803952942Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG125350.20944163211756936Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG122630.2048969074318112Clontech SMART CDS Primer II A (100% over 26bp)
GCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98120.16394425962007103Clontech SMART CDS Primer II A (100% over 24bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA76760.12825480399955821Clontech SMART CDS Primer II A (100% over 26bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTA75430.1260325672965956Clontech SMART CDS Primer II A (100% over 26bp)
AGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68050.11370165987714875Clontech SMART CDS Primer II A (100% over 25bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG67390.11259889579898683No Hit
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG61030.10197226013669931Clontech SMART CDS Primer II A (100% over 26bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)